DyLight® Dyes
DyLight dyes give you outstanding immunofluorescence results in flow cytometry and microscopy.
- More intense fluorescence and sensitivity than comparable spectral conjugates
- Greater photostability than other fluorochromes
- Good stability over pH 4 - 9
- Ideal for immunofluorescence techniques
- Compatible with all common fluorescence detection channels
- Available for custom conjugation
DyLight 405
DyLight 488
DyLight 549
DyLight 649
DyLight 680
DyLight 750
DyLight 800
DyLight dyes are excellent alternatives to Alexa Fluor®, CyDye® and LI-COR® fluorescent dyes. DyLight dyes provide intense fluorescence and sensitivity requiring less conjugated antibody to study your cells than FITC and RPE, saving you money.
They are primarily suitable for use in immunofluorescence techniques such as microscopy and flow cytometry though may also be used in ELISA, Western blotting, high throughput screening and other array platforms.
Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation, so you won’t have to change your preferred method of imaging.
We currently offer the following DyLight dyes conjugated to primary antibodies and secondary antibodies, and they are also available for custom conjugations to the antibody of your choice.
| Dye | Laser excitation line (nm) |
Maximal absorbance (nm) | Maximal emission (nm) | Suggested laser | Fluores- cence color |
|---|---|---|---|---|---|
| DyLight® 405 | 400 | 405 | 420 | Violet diode | |
| DyLight® 488 | 493 | 488 | 518 | Argon-Ion | |
| DyLight® 549 | 550 | 549 | 568 | Krypton-Argon | |
| DyLight® 649 | 646 | 649 | 674 | Krypton-Argon | |
| DyLight® 680 | 682 | 680 | 715 | Argon-Ion | |
| DyLight® 750 | 752 | 750 | 778 | Argon-Ion | |
| DyLight® 800 | 770 | 800 | 794 | Argon-Ion |
DyLight 405
- Exceptionally resistant to photo-bleaching and is an ideal blue fluorophore for demanding fluorescence conditions
- Equivalent to Alexa Fluor® 405 and Cascade blue dyes
DyLight 405 Dye: Absorption (black), Emission (blue)
DyLight 488
- Outperforms FITC and Cy2 with fluorescent brightness, photostability and pH stability
- Equivalent to Alexa Fluor® 488, fluorescein and FITC dyes
DyLight 488 Dye: Absorption (black), Emission (green)
DyLight 549
- Has a bright and more photostable fluorescence than Cy3 dye
- Equivalent to Alexa Fluor® 546, Alexa Fluor® 555, Cy3 and TRITC dyes
DyLight 549 Dye: Absorption (black), Emission (yellow)
DyLight 649
- DyLight 649 closely matches Cy5 spectral properties with brighter and more photostable fluorescence
- Equivalent to Alexa Fluor® 647 and Cy5
DyLight 649 Dye: Absorption (black), Emission (red)
DyLight 680
- DyLight 680 is a superior alternative to Cy5.5
- Can be separated from Texas Red or Rhodamine
- Equivalent to Alexa Fluor® 680, Cy5.5
- Compatible for use in the LI-COR® Odyssey Infrared Imaging System
DyLight 680 Dye: Absorption (black), Emission (brown, near IR)
DyLight 750
- Equivalent to Alexa Fluor® 750
- Higher fluorescence and photostability than LI-COR Dyes
- Compatible for use in the LI-COR® Odyssey Infrared Imaging System
DyLight 750 Dye: Absorption (black), Emission (brown, near IR)
DyLight 800
- Outperforms IRDye® 800 with extremely low autofluorescence – ideal for in vivo imaging
- Compatible for use in the LI-COR® Odyssey Infrared Imaging System
DyLight 800 Dye: Absorption (black), Emission (brown, infrared)
We offer custom conjugations of these DyLight dyes to any of our primary antibodies and to your own antibody. Not all antibody suppliers can offer you this Custom Service; and this is just one of the many ways AbD Serotec tries harder to meet your specific antibody needs.
Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.
CyDye® is a trademark of GE Healthcare.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
LI-COR® and IRDye® 800 are trademarks of LI-COR Biosciences.
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